Kane will miss both legs of Champions League last 16 with Borussia Dortmund.
Harry Kane’s Spurs short of options up front, with Son Heung-Min also unavailable.
He’ll also be out for the Carabao Cup final, on February 24, should Spurs make it.
Kane faces a race to be fit for England’s Euro 2020 qualifiers in late March.
It is possible Kane will miss 13 fixtures in a major blow to Tottenham’s season.
Harry Kane faces at least two months out after damaging ankle ligaments in Tottenham’s home defeat by Manchester United on Sunday.
Spurs confirmed the news on Tuesday afternoon that Kane will not be able to train again until early March, so will be unlikely to play again until later that month.
A club statement read: ‘Following preliminary assessments, we can confirm that Harry Kane has damaged ligaments in his left ankle, sustained during Sunday’s match.
SPURS GAMES HARRY KANE COULD MISS
January 20 – Fulham (A) Premier League
January 24 – Chelsea (A) Carabao Cup
January 27 – Crystal Palace (A) FA Cup
January 30 – Watford (H) Premier League
February 2 – Newcastle (H) Premier League
February 10 – Leicester (H) Premier League
February 13 – Borussia Dortmund (H) Champions League
February 23 – Burnley (A) Premier League
February 24 – Carabao Cup final
February 27 – Chelsea (A) Premier League
March 2 – Arsenal (H) Premier League
March 5 – Borussia Dortmund (A) Champions League
March 9 – Southampton (A) Premier League
March 16 – Crystal Palace (H) Premier League
‘He will continue to be monitored by our medical staff as he commences rehabilitation and is expected to return to training in early March.’
It leaves Tottenham devoid of options up front, with Son Heung-Min also set to miss five upcoming games while on international duty for South Korea at the Asian Cup.
Kane will miss both legs of Spurs’ Champions League last-16 meeting with Borussia Dortmund, and the Carabao Cup semi-final second leg against Chelsea. He will also be out for the final, on February 24, should Tottenham make it.
The striker is set to miss as many as nine Premier League fixtures, including London derbies against Chelsea and Arsenal at the end of February and start of March respectively.
It is possible Kane will miss 14 fixtures, which would be his longest-ever absence due to injury.
England manager Gareth Southgate will also be sweating on his captain’s fitness, with their first two Euro 2020 qualifiers – at home to Czech Republic and away at Montenegro – on March 22 and 25 respectively.
Kane will also be stretching to be fit for Spurs’ home game against Crystal Palace on March 16 – a fixture pencilled in for possibly being the first played at the refurbished White Hart Lane.
His injury leaves Tottenham painfully short of strikers – Fernando Llorente and the out-of-favour Vincent Janssen are their only fit, senior centre forward options.
With Son also unavailable until early February, the likes of Erik Lamela or Lucas Moura could be press-ganged into action up front.
It is the latest in a series of ankle injuries for Kane, the fourth in his career. The worst came in September 2016, where he was also out for two months and missed 11 matches.
Kane suffered the blow to the ankle after a hefty tackle by England team-mate Phil Jones.